This event is being held onWednesday 11th February 2009 at The Chandelier Suite, The Assembly Rooms,

Fenkle Street, Newcastle-Upon-Tyne, NE1 5XU.

 

Organisers: Ian Dimmick and Rebecca Stewart (ian.dimmick@ncl.ac.uk; rebecca.stewart@ncl.ac.uk)

 

 

Sessions are suitable for all levels and will begin with basics and then expand into the more complex aspects and practicalities of flow cytometry.

 

 

PROGRAMME:

 

 

9.00 -9.20am Refreshments

 

9.20-9.30 Welcome

 

Introduction to instrumentation

 

9.30-10.00 Becton Dickinson instrumentation and quality control (Instrumentation layout, fluidics, filters and photodetectors) - M Dyson (Becton Dickinson)

 

1000-1030 Beckman Coulter instrumentation and quality control (Instrumentation layout, fluidics, filters and photodetectors) - M Cheetham (Beckman Coulter)

 

1030-1100 Coffee and trade exhibition

 

 

Experimental setup and multicolour flow cytometry

 

1100-1130 Fluorescence, fluorochromes and isotype controls (Fluorescence explained, fluorochrome choice, antibody titration, isotype controls) - I Dimmick (Newcastle University)

 

1130-1200 Multicolour flow cytometry and compensation (Spectral overlap explained, the dos and don’ts of compensation, problems and solutions) - R Stewart (Newcastle University)

 

 

Fluorescence-activated cell sorting

 

1200-1245 Cell sorting: An overview - J Lawry (Becton Dickinson)

 

1245-1400 Lunch and trade exhibition

 

Data analysis

 

1400-1430 Flow cytometric data analysis - C Vallan (FlowJo, Treestar Inc.)

 

1430-1500 FCS File Structure & Analysis Using VenturiOne - P Nobes (Applied Cytometry)

 

1500-1530 Coffee and trade exhibition

 

 

Techniques and applications

 

1530-1600 Flow cytometric analysis of the cell cycle and cell proliferation - Annemieke Walker (Invitrogen)

 

1600-1630 Measurement of telomere length in white blood cells by flow cytometry - I Spyridopoulos (Cardiology, Newcastle University)

 

1630-1700 Cytometric assessment of apoptotic cell death - D Davies (CRUK)

 

1700-1730 Flow cytometry and fluorescence-activated cell sorting of the human dermis - M Haniffa (Academic Haematology, Newcastle University)

 

1730-1745 Closing remarks

 

Conference fee: £25 per delegate (Lunch and refreshments will be provided). If you would like to attend or find out more information, please email  Ian Dimmick (ian.dimmick@ncl.ac.uk) or  Rebecca Stewart (rebecca.stewart@ncl.ac.uk). 

 

Please note: All delegates are also invited to attend the FlowJo Seminar with Claudio Vallan (FlowJo, TreeStar Inc.)

on Tuesday 10th February, 4:30pm (Institute of Human Genetics, Newcastle-Upon-Tyne, NE1 3BZ). This seminar is complimentary for all delegates